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Advances in improving mammalian cells metabolism for recombinant protein production Electron. J. Biotechnol.
Altamirano,Claudia; Berrios,Julio; Vergara,Mauricio; Becerra,Silvana.
Background: The production of recombinant proteins for therapeutic use represents a great impact on the biotechnology industry. In this context, established mammalian cell lines, especially CHO cells, have become a standard system for the production of such proteins. Their ability to properly configure and excrete proteins in functional form is an enormous advantage which should be contrasted with their inherent technological limitations. These cell systems exhibit a metabolic behaviour associated with elevated cell proliferation which involves a high consumption of glucose and glutamine, resulting in the rapid depletion of these nutrients in the medium and the accumulation of ammonium and lactate. Both phenomena contribute to the limitation of cell...
Tipo: Journal article Palavras-chave: Aerobic glycolysis; Cell engineering; CHO cells; Glutaminolysis; Metabolism.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300010
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Maternal transmission of mitochondrial diseases Genet. Mol. Biol.
Chiaratti,Marcos R.; Macabelli,Carolina H.; Augusto Neto,José Djaci; Grejo,Mateus Priolo; Pandey,Anand Kumar; Perecin,Felipe; Collado,Maite del.
Abstract Given the major role of the mitochondrion in cellular homeostasis, dysfunctions of this organelle may lead to several common diseases in humans. Among these, maternal diseases linked to mitochondrial DNA (mtDNA) mutations are of special interest due to the unclear pattern of mitochondrial inheritance. Multiple copies of mtDNA are present in a cell, each encoding for 37 genes essential for mitochondrial function. In cases of mtDNA mutations, mitochondrial malfunctioning relies on mutation load, as mutant and wild-type molecules may co-exist within the cell. Since the mutation load associated with disease manifestation varies for different mutations and tissues, it is hard to predict the progeny phenotype based on mutation load in the progenitor. In...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oocyte; Germline; Mitochondrial dynamics; MtDNA; Metabolism.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200308
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Purine and pyrimidine nucleotide metabolism in Mollicutes Genet. Mol. Biol.
Bizarro,Cristiano Valim; Schuck,Desirée Cigaran.
Several mollicute genome projects are underway, offering unique opportunities to study genes and metabolic pathways on a genome-wide scale. Here, we have analyzed the conservation and diversity of purine and pyrimidine metabolism in mycoplasmas. An evaluation of discrepancies between genomic analysis and enzymatic data revealed interesting aspects about these organisms. We found important examples in which enzyme activity was reported without the annotation of a corresponding gene. An interesting example concerns phosphopentomutase. In Mollicutes, we have identified CDSs orthologous to sequences recently identified as new phosphopentomutases in archaeobacteria that are structurally related to phosphomannomutases. It is suggested that these sequences could...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mollicutes; Purine; Pyrimidine; Metabolism; Metabolic pathways.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200005
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Gender-dependent association of HSD11B1 single nucleotide polymorphisms with glucose and HDL-C levels Genet. Mol. Biol.
Turek,Luciane Viater; Leite,Neiva; Souza,Ricardo Lehtonen Rodrigues; Lima,Jovana Karoline; Milano,Gerusa Eisfeld; Timossi,Luciana da Silva; Osiecki,Ana Claudia Vecchi; Osiecki,Raul; Alle,Lupe Furtado.
In this study, we investigated the influence of two SNPs (rs846910 and rs12086634) of the HSD11B1 gene that encodes 11β-hydroxysteroid dehydrogenase type 1(11β-HSD1), the enzyme that catalyzes the conversion of cortisol to cortisone, on variables associated with obesity and metabolic syndrome in 215 individuals of both sexes from southern Brazil. The HSD11B1 gene variants were genotyped using the TaqMan SNP genotyping assay. Glucose, triglycerides, total cholesterol, HDL-cholesterol and LDL-cholesterol were measured by standard automated methods. Significant results were found in women, with carriers of the G allele of SNP rs12086634 having higher glucose levels than non-carriers. Carriers of the A allele of SNP rs846910 had higher levels of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HSD11B1 gene; Men; Metabolism; Metabolic syndrome; Women.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400003
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Metabolic adjustments in Satanoperca aff. jurupari (Perciformes: Cichlidae) Genet. Mol. Biol.
Chippari-Gomes,Adriana R.; Leitão,Marco Aurélio B.; Paula-Silva,Maria de Nazaré; Mesquita-Saad,Lenise S. B. de; Almeida-Val,Vera Maria F..
In this paper, we describe the enzyme levels and isozyme distribution in skeletal and heart muscle of Satanoperca aff. jurupari, (Cichlidae, subgroup Geophaginae). LDH and CS were measured in skeletal and heart muscle. Starch gel electrophoresis was used to determine the isozyme/allozyme patterns in different tissues; LDH, MDHs, PGM, PGI, ADH, G-6-PDH and SOD were screened for the numbers of loci, presence of alleles, and tissue specificity. The LDH/CS ratio in heart and skeletal muscle were 173.36 and 6.1, respectively, indicating anaerobic metabolism in the former and aerobic metabolism in the latter muscle. No inhibition by pyruvate (based on the ratios of LDH activity with 1 mM and 10 mM pyruvate) was detected in heart and skeletal muscle, indicating...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cichlid; Plasticity; Metabolism; Enzymes; Amazon.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100005
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Using the FORESTS and KEGG databases to investigate the metabolic network of Eucalyptus Genet. Mol. Biol.
Mombach,José C.M.; Lemke,Ney; Silva,Norma M. da; Ferreira,Rejane A.; Isaia Filho,Eduardo; Barcellos,Cláudia K.; Ormazabal,Rodrigo J..
In this work we apply a bioinformatics approach to determine the most important enzymes of the metabolic network of Eucalyptus to determine the coverage of the genome in the FORESTS library. We conclude that the library does not cover completely the metabolism of the organism. However, some important pathways could be analyzed, especially the lignin synthesis. We found that four of the most important enzymes predicted are involved in this pathway.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Eucalyptus; Metabolism.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400018
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Genome-wide comparison of genes involved in the biosynthesis, metabolism, and signaling of juvenile hormone between silkworm and other insects Genet. Mol. Biol.
Cheng,Daojun; Meng,Meng; Peng,Jian; Qian,Wenliang; Kang,Lixia; Xia,Qingyou.
Juvenile hormone (JH) contributes to the regulation of larval molting and metamorphosis in insects. Herein, we comprehensively identified 55 genes involved in JH biosynthesis, metabolism and signaling in the silkworm (Bombyx mori) as well as 35 in Drosophila melanogaster, 35 in Anopheles gambiae, 36 in Apis mellifera, 47 in Tribolium castaneum, and 44 in Danaus plexippus. Comparative analysis showed that each gene involved in the early steps of the mevalonate (MVA) pathway, in the neuropeptide regulation of JH biosynthesis, or in JH signaling is a single copy in B. mori and other surveyed insects, indicating that these JH-related pathways or steps are likely conserved in all surveyed insects. However, each gene participating in the isoprenoid branch of JH...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Juvenile hormone; Biosynthesis; Metabolism; Signaling; Gene duplication.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300018
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Differential metabolism of Mycoplasma species as revealed by their genomes Genet. Mol. Biol.
Arraes,Fabricio B.M.; Carvalho,Maria José A. de; Maranhão,Andrea Q.; Brígido,Marcelo M.; Pedrosa,Fábio O.; Felipe,Maria Sueli S..
The annotation and comparative analyses of the genomes of Mycoplasma synoviae and Mycoplasma hyopneumonie, as well as of other Mollicutes (a group of bacteria devoid of a rigid cell wall), has set the grounds for a global understanding of their metabolism and infection mechanisms. According to the annotation data, M. synoviae and M. hyopneumoniae are able to perform glycolytic metabolism, but do not possess the enzymatic machinery for citrate and glyoxylate cycles, gluconeogenesis and the pentose phosphate pathway. Both can synthesize ATP by lactic fermentation, but only M. synoviae can convert acetaldehyde to acetate. Also, our genome analysis revealed that M. synoviae and M. hyopneumoniae are not expected to synthesize polysaccharides, but they can take...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mollicutes; Mycoplasma; Metabolism; Comparative genomics.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200004
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Acephate and methamidophos residues in greenhouse and in field grown tomatoes Horticultura Brasileira
Trevizan,Luiz Roberto P.; Baptista,Gilberto C. de; Papa,Geraldo.
Increasing horticultural production under protected cultivation requires more detailed studies about the occurence of pesticide residues, due to the special and peculiar environment under controlled conditions, in which some pests, that are of little concern in the field, become important. The insecticide acephate and its methamidophos metabolite residues were evaluated in greenhouse-grown tomatoes and compared to an open-field tomato crop. The treatments: (a) check; (b) one application of 75 g a.i. acephate.100 L-1 water; (c) one application of 150 g a.i.100 L-1 water; (d) four applications of 75 g a.i.100 L-1 water were evaluated. Fruit, leaf and soil samples were taken at (-1), zero, 1, 3, 7, 14, 21 days after the last or only application. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycopersicon esculentum; Pesticide residues; Metabolism; Greenhouse.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362005000100008
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Энергетический баланс и чистая эффективность роста плоской устрицы (Ostrea edulis L.) IBSS Repository
Золотницкий, А. П.; Сытник, Н. А..
Исследованы энергетический баланс и чистая эффективность роста (коэффициент К2) плоской устрицы (Ostrea edulis L.) в онтогенезе. Выявлены общие тенденции изменений отдельных его компонентов в процессе роста и распределение энергетических трат на индивидуальную продукцию, энергетический обмен, ассимилированную и потреблённую пищу. Дана характеристика сезонных изменений чистой эффективности роста, связанных с температурой воды и массой тела, динамика которых описывается уравнением множественной регрессии. Показано, что максимальные значения К2 у исследованного вида могут составлять 0.63 – 0.67.
Tipo: Journal Contribution Palavras-chave: Устрица; Энергетический баланс; Рост; Метаболизм; Ассимиляция; Рацион; Эффективность; Устриця; Енергетичний баланс; Зростання; Метаболізм; Асиміляція; Раціон; Ефективність; Oyster; Energy balance; Growth; Metabolism; Assimilation; Ration; Efficiency.
Ano: 2010 URL: http://repository.ibss.org.ua/dspace/handle/99011/1919
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Seasonal variation of the effect of high-carbohydrate and high-protein diets on the intermediate metabolism of Parastacus brasiliensis (Crustacea, Decapoda, Parastacidae) maintained in the laboratory Iheringia, Sér. Zool.
Dutra,Bibiana K.; Silva,Karina M. da; Zank,Caroline; Conter,Maria R.; Oliveira,Guendalina T..
The goal of this study was to evaluate the effect of a high-carbohydrate diet (HC) and a high-protein diet (HP) on the metabolism of the crayfish Parastacus brasiliensis (Von Martens, 1869), collected in different seasons and maintained in the laboratory for 15 days. Crayfish were collected monthly from January 2002 to January 2004 at São Francisco de Paula, Southern Brazil, in Guarapirá stream. In the laboratory, the animals were kept submerged in aquariums under controlled conditions. They were fed ad libitum, for 15 days with either a HC or HP diet. At the end of this period, haemolymph samples were collected, as were hepatopancreas, gills, and abdominal muscle that were removed for determination of glycogen, free glucose, lipids, and triglycerides. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crayfish; Experimental culture; Diet; Metabolism; Seasonality.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212008000400003
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Physiological responses of anti-predation in prey fish to the threat of piscivorous fish in different underwater visibility conditions Iheringia, Sér. Zool.
Fiori,Leandro Fabrício; Figueiredo,Bruno R. S.; Pavanello,Audrei; Alves,Vander Silva; Mathias,Paulo Cezar de Freitas; Benedito,Evanilde.
ABSTRACT Considering that the anti-predation behaviour of prey fishes may vary with predator hunting strategy, we experimentally investigated the physiological responses of Astyanax bimaculatus (Linnaeus, 1758) shoals to the presence of two piscivorous fish species with different hunting modes: sit-and-wait and active pursuit. In addition, we evaluated the influence of underwater visibility conditions on the interaction between predator and prey. We tested the hypotheses that: (i) prey plasma cortisol and glucose levels vary according to piscivore hunting strategy, and (ii) no reduction in plasma glucose and cortisol levels takes place in turbid environments due to the inability of prey to accurately recognise predators by non-visual signals. The results...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metabolism; Hoplias aff. malabaricus; Astronotus crassipinnis; Astyanax bimaculatus; Turbidity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212018000100232
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Transferência de supressividade de solos a Rhizoctonia solani e efeito de metabolismos voláteis. Infoteca-e
GHINI, R..
A possibilidade de transferencia da supressividade a R. solani GA 4 foi testada atraves da mistura de um solo supressivo com um solo conducente nas concentracoes de 0; 0,1; 0,5; 1; 5; 10 e 100%. O aumento da concentracao do solo supressivo resultou numa reducao do crescimento micelial do patogeno. No houve transferencia da supressividade apos o tratamento biocida do solo (fumigacao com cloroformio), comprovando o efeito dos microrganismos na supressividade. A presenca de metabolitos volateis foi outro mecanismo de supressividade observado.
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Solo supressivo; Metabolismo; Queima da Saia; Rhizoctonia Solani; Solo; Metabolism; Soil.
Ano: 2000 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1111024
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Defasagem entre as produtividades real e potencial do algodoeiro herbáceo: limitações morfológicas, fisiológicas e ambientais. Infoteca-e
BELTRÃO, N. E. de M.; AZEVEDO, D. M. P. de.
Fatores limitantes relacionados com a morfologia, a organografia e arquiteture do algodoeiro herbaceo; fatores limitantes relacionados com -a fisiologia e a bioquimica da planta do algodoeiro herbaceo; fatores limitantes relacionados com as condicoes do ambiente; consideracoes holoticas envolvendo fatores da planta e do ambiente.
Tipo: Livro técnico (INFOTECA-E) Palavras-chave: Herbaceo; Taxas de Crescimento; Cultura; Fotorrespiracao; Trofofilos; Dossel; Balanco; Assimilado; Limitacao incontrolavel; Desenvolvimento; Brasil; Paraiba; Annual cotton; Production; Growth rate; Reespiration; Assimilation; Crop; Levll; Not controllable; Limitation; Growth; Deelopment.; Algodão; Bioquímica; Crescimento; Fotossíntese; Fisiologia Vegetal; Gossypium Hirsutum; Metabolismo; Respiração.; Produção; Biochemistry; Canopy; Leaves; Physiology.; Photosynthesis; Photorespiration; Metabolism.
Ano: 1993 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/264934
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Ação hormonal da leptina em ruminantes. Infoteca-e
SALMAN, A. K. D.; COSTA, R. B..
A leptina, chamada de hormônio da obesidade, é produzida e secretada pelos adipócitos. É um hormônio capaz de alterar o consumo de alimento e o gasto de energia, por parte do animal, pois informa o cérebro sobre a quantidade de energia armazenada em forma de gordura. Propõe-se, nesta Documentos, explicar a ação da leptina em ruminantes. O trabalho apresenta os seguintes tópicos: Polimorfismos no gene da leptina; Expressão gênica da leptina; Os receptores da leptina; Controle do apetite pela leptina; Modulação fisiológica da leptina; Ação da leptina sobre a reprodução.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Leptina; Animal hormone.; Bovino; Hormônio Animal.; Metabolismo Animal; Metabolism; Cattle; Leptin..
Ano: 2006 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/710735
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The planktonic stages of the salmon louse ( Lepeophtheirus salmonis) are tolerant of end-of-century p CO2 concentrations ArchiMer
Thompson, Cameron R.s.; Fields, David M.; Bjelland, Reidun M.; Chan, Vera Bin San; Durif, Caroline M.f.; Mount, Andrew; Runge, Jeffrey A.; Shema, Steven D.; Skiftesvik, Anne Berit; Browman, Howard I..
The copepod Lepeophtheirus salmonis is an obligate ectoparasite of salmonids. Salmon lice are major pests in salmon aquaculture and due to its economic impact Lepeoph- theirus salmonis is one of the most well studied species of marine parasite. However, there is limited understanding of how increased concentration of pCO2 associated with ocean acidification will impact host-parasite relationships. We investigated the effects of increased pCO2 on growth and metabolic rates in the planktonic stages, rearing L. salmonis from eggs to 12 days post hatch copepodids under three treatment levels: Control (416 matm), Mid (747 matm), and High (942 matm). The pCO2 treatment had a significant effect on oxygen consumption rate with the High treatment animals exhibiting...
Tipo: Text Palavras-chave: Salmon lice; Copepod; Ocean acidification; Parasite; Energetics; Metabolism; Growth; Lipid; Lepeophtheirus salmonis; Aquaculture.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00588/69998/67911.pdf
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Effets d’une alimentation végétale sur la biosynthèse des acides gras et le métabolisme du bar européen (Dicentrarchus labrax) ArchiMer
Geay, Florian.
In the context of the fish farming industry needing to substitute the animal food (meal and oil) by vegetable ingredients, the main objective of this thesis was to study the long-term effects of a diet exclusively composed by plant products on the metabolism of the european sea bass (Dicentrarchus labrax) and more particularly, on its capacity to biosynthesis the Highly Unsaturated Fatty Acids (HUFA) from the PolyUnsaturated Fatty acids (PUFA) brought by this vegetal diet. Our transcriptomic approaches showed that the use of a plant food disrupted the lipids metabolism (cholesterol and fatty acids) and the energy metabolism (carbohydrates, amino acids) in the liver, but also affected other physiological processes such as the immune system. In spite of the...
Tipo: Text Palavras-chave: Aquaculture; Nutrition; Métabolisme; Lipides; Désaturase; Delat-6-désaturase (D6D); Huile végétale; Farine végétale; Acides gras à longues chaînes carbonées polyinsaturés (AGLPI); Aquaculture; Nutrition; Metabolism; Lipids; Desaturase; Fatty acids desaturase 2 (fads2); Vegetable oil; Vegetable meal; Highly unsaturated fatty acids (HUFA).
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00048/15925/13352.pdf
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Seasonal and diurnal variability in carbon respiration, calcification and excretion rates of the abalone Haliotis tuberculata L. ArchiMer
Chapperon, Coraline; Clavier, Jacques; Dugué, Clément; Amice, Erwan; Le Goff, Marion; Roussel, Sabine.
Abalone (Haliotis spp.) are commercially important marine shellfish species worldwide. Knowledge about the physiology of abalone that impacts life-history traits is important for a better understanding of the biology of the species and the impact of stressful husbandry procedures at different seasons. The present study quantified the seasonal and diurnal variations in four physiological parameters of the European species Haliotis tuberculata, i.e. carbon aerial and aquatic respiration, calcification and excretion rates, and the effect of prolonged aerial exposure upon abalone aerial respiration. We also investigated the effect of individual size upon these physiological parameters. Aquatic respiration and calcification rates showed an allometric...
Tipo: Text Palavras-chave: Ormer; Metabolism; Carbon fluxes; Physiology.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00431/54243/55886.pdf
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Zooplankton metabolism in South African estuaries: does habitat type influence ecological strategies? ArchiMer
Tagliarolo, Morgana; Porri, F; Garvie, C D; Lechman, K; Scharler, U M.
Zooplankton community composition, biomass and metabolism can vary drastically over space and time in subtropical estuaries. Changing environmental conditions can affect communities differently, depending on the characteristics of the species involved. In the present study, we compared the rates of oxygen consumption of the dominant zooplankton taxa living in permanently open and in temporally open/closed estuaries. The metabolic response was tested at four temperatures experienced by the animals in their natural environment. Zooplankton from the temporally open/closed estuary showed low activation energies and low rate of metabolism at the highest temperature tested. Animals from the permanently open estuary had higher respiration rates at increased...
Tipo: Text Palavras-chave: Metabolism; Zooplankton; Respiration; Estuary; Thermal sensitivity.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00513/62425/66904.pdf
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Experimental shift in diet Delta13C: A potential tool for ecophysiological studies in marine bivalves ArchiMer
Paulet, Yves-marie; Lorrain, Anne; Richard, Joëlle; Pouvreau, Stephane.
To test the potential of diet switching experiments in ecophysiological studies of marine invertebrates, stable carbon isotope ratios were measured at different seasons in the gonad, adductor muscle, digestive gland and gills of scallops (Pecten maximus) and oysters (Crassostrea gigas) held for 15 days on a constant diet of phytoplankton depleted in 13C. The aim of this study was to determine if differences in carbon incorporation could be detected among species, seasons and organs, and if so, whether it was consistent with their known energy-allocation patterns. After offering the new diet, isotope values of the different organs gradually shifted and significant differences among organs, seasons and species were found. A carbon incorporation index (CII)...
Tipo: Text Palavras-chave: Crassostrea gigas; Pecten maximus; Carbon isotopes; Metabolism; Energy allocation.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1202.pdf
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